Journal: Journal of Ovarian Research

Article Title: Long-term 3D culture of human cumulus granulosa cell spheroids in PEGylated fibrin: a preclinical model for reproduction and ovarian research

doi: 10.1186/s13048-026-02079-w

Figure Lengend Snippet: Experimental workflow for hCGC spheroid culture. Schematic representation of the experimental process: COCs were obtained from IVF/ICSI patients at Saint-Luc University Hospital and treated with hyaluronidase to isolate CGCs. Isolated CGCs were seeded in ultra-low attachment plates to form spheroids over 6 days, followed by encapsulation in PEGylated fibrin hydrogel for long-term 3D culture (up to 30 days). Created with BioRender.com

Article Snippet: Cells were seeded at a density of 2000 cells per well in 96-well ultra-low attachment plates (83.3925.400, Sarstedt, Nümbrecht, Germany) and cultured for 6 days at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Isolation, Encapsulation